Production of Gamma-Aminobutyric Acid by Levilactobacillus brevis CD0817 by Coupling Fermentation with Self-Buffered Whole-Cell Catalysis

There is a recent trend of using lactic acid bacteria for the production gamma-aminobutyric (GABA). This study described method that combines fermentation and self-buffered whole-cell catalysis efficient GABA Levilactobacillus brevis CD0817. Upon completion fermentation, cells were recovered to conduct by which substrate L-glutamic was catalytically decarboxylated GABA. itself maintained acidity essential decarboxylation. To maximize ability, effects cell culture method, temperature, time, concentration, dosage investigated. The results illustrate cultivated 16 h in medium supplemented with 20.0 g/L glucose most suitable catalytic At h, fermentative content reached 204.2 g/L. Under optimized conditions (temperature 45.0 °C, time 12.0 wet 25.0 g/L, 120.0 g/L), 85.1 obtained, 3.7 ± 0.9 residue. from system sequentially performing rotary vacuum evaporation, precipitation ethanol, filtration filter paper, drying. purity product 97.1%, recovery rate 87.0%. These data suggest proposed has potential applications